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The expressions of the PRV genes were analyzed, categorized and compared by qRT2-PCR assay, with the average of the minimum cycle threshold used as a control for the calculation of a particular R value.
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AIV, avian influenza virus; Ct, cycle threshold value; HA, hemagglutinin; NA, neuraminidase; min, minimum; max, maximum; ND, not determined.
Cycle threshold (Ct) values were determined for a minimum of three biological replicates and based on three technical replicates.
The first method was based on cycle threshold and Cq determinations, according to the guidelines of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments[ 18, 19], and the other method used the relative threshold algorithm of the ViiA 7 software to determine the cycle relative threshold (CRT) of each sample.
According to the product monograph, the amplification curve of the Xpert® C. difficile assay must have a cycle threshold within a valid range and endpoint above a set minimum to be considered positive for tcdB.
Cycle Threshold.
The minimum cycle mean of the graph is the minimum mean weight of a cycle.
Cycle threshold (Ct) values were recorded.
The cycle threshold (CT) was calculated automatically.
Ct, cycle threshold.
Ct, cycle threshold; –, negative; +, positive.
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