Sentence examples for minimum cycle number from inspiring English sources
PCR cycle optimization was performed to determine the minimum cycle number to amplify full-length cDNA products using the SMART primers and Clontech Advantage-HF 2 polymerase Mix (Clontech/Takara Bio, Mountain View, CA).
Reactions were incubated at 98° for 30 sec, followed by 17 21 cycles of the following: 98° for 10 sec, 63° for 30 sec, and 72° for 1.5 min. Reactions were amplified for the minimum cycle number required to produce a visible product on a 1% agarose gel.
Then, the flow number at the minimum point of new strain rate versus cycle number was determined.
A common method in examining the flow number is to locate the lowest point in the strain rate versus cycle number curve, or the minimum value of the strain rate.
Five factors were considered, the five impedance spectroscopy measurement parameters: maximum integration time; minimum number of integration cycles; number of stabilization cycles; maximum stabilization time; and minimum cycle fraction.
For instance, by monitoring library amplification in real time, cycle number can be kept to a minimum while still ensuring that the reaction has reached the exponential phase and produced enough DNA for the final library.
Karp gave a characterization of minimum cycle mean and an O(nm) algorithm to compute it, where n is the number of vertices and m is the number of edges.
We calculated the values of a behavior spectrum at 64 time steps of a cycle ranging from 0.05 to 1 sec as: <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0005379.e007.PNG" class= inline-graphic"/> where T is the number of time steps (64 steps), Cmin is the minimum cycle (0.05 sec), and Cmax is the maximum cycle (1 sec).
Figure 8 Performance of computing minimum cycle basis.
A minimum cycle basis is a cycle basis of minimum weight, in an unweighted graph the weight is simply the number of edges.
