Note that both EEL and CONREAL failed to detect about 40 60% of true positive STAT3 sites even at the minimum cut-off value, while STAT-Finder found all of these.
A minimum cut-off value of 100bp was applied after quality control processing for generating the submission file for EMBL (Accession numbers, FM897377– FM897377).
Several of the remaining 13 genes (e.g. YBL048W, COS4, COS8, RAD27) showed increased expression in Zap1TC-expressing cells although these effects did not meet our minimum cut-off value of 1.5-fold changes (Additional file 1).
Many of these sequences had low RSAT scores near the minimum cut-off value of 4.5 and were very divergent from the consensus ZRE sequence (ACCTTNAAGGT, RSAT score = 11.7) (Fig. 1B).
An RSAT score of 4.5 was used as a minimum cut-off value in this analysis because this is the lowest score obtained for a bona fide ZRE found in the EKI1 promoter [ 21].
A minimum cut-off value of 150 bp was applied after quality control processing for sequence database searching and for generating the submission file for dbEST [ 48] (Accession numbers, dbEST: 49109381 49125759, Genbank: EW744731– EW744731).
The minimum cut-off values of the IRAR index for insulin resistance assessment were between 3.538 and 3.955.
The minimum cut-off values of the novel IRAR index for insulin resistance assessment in Malaysian men were between 3.538 and 3.955.
The minimum cut-off values of the novel AR index for diagnosis of T2DM and MS in Malaysian men were between 1.206 and 1.244.
Although a booster was not included in this study, the vaccine was immunogenic (seroprotection above accepted minimum cut-off values) following the primary vaccination course.
Therefore, further investigation or replicate studies are required to validate the suggestive reference range or minimum cut-off values of insulin sensitivity for the IRAR index in Malaysian men.
