The minimum cut off for the identification of mono-, di-, tri-, tetra-, penta- and hexanucleotide SSRs was set at 10, 6, 5, 5, 5 and 5 repeats, respectively.
An NR event was tallied when a distinct three-dimentional invagination of the envelope into the nuclear space was identified, with a minimum size cut off of 1 μm; superficial wrinkles and folds of the nuclear envelope were not considered.
These two data sets were used to mine the protein sets of each sequenced Lachnospiraceae genome using USEARCH 4.0.38 (Edgar 2010) with an e-value cut off of 10− and a minimum identity cut off of 70%.
‡Discarded reads were removed using Trimmomatic based on a sliding window quality cut off of (4:15) and a minimum length of 36 nt after adapter removal.
The commonly accepted cut off of 0.15 for the pairwise variation was used to assess the minimum number of reference genes required for accurate normalization [ 9].
(E.g., if the body height of Americans was power-law distributed with minimum cut-off of 4'5 and average 5'7, then there would be about 40,000 Americans taller than the Empire State Building).
Consequently, our filtering was set to a minimum cut-off of 10 X CCS coverage depth.
Mannan testing for the diagnosis of Candida infections was reported in two studies, each using a minimum cut-off of 0.5 ng/ml [ 56, 79].
All H&E stained slides were reviewed by a pathologist to define the percentage of tumour with a minimum cut-off of >70%.
This paper was relatively old and used considerably higher hypertension cut-offs than more contemporary work (variable with minimum cut-off of 160/100mm Hg), which would be expected to result in lower detection rates given a similar population.
