Sentence examples for minimum coverage thresholds from inspiring English sources

Candidate gene duplicate pairs obtained in the above GH analyses were also filtered using minimum coverage thresholds, where query and subject sequences were required to show at least 70% coverage, prior to forming single-linkage clustering gene families.

Calculating the probability of NCO GCs per marker at a series of minimum coverage thresholds revealed that beyond a minimal coverage of 10, the frequency of NCO GC remained stable.

Lowering the minimum coverage threshold to 50%% increased mapping by between 2 % and 15%%.

For the purposes of the current study, a minimum coverage threshold of 5× was used for STR allele determination.

Additionally, non-specific matches and broken pairs were ignored, and the program enforced a 20-fold minimum coverage threshold and 90% minimum variant frequency.

In order to distinguish between homozygous and heterozygous genotypes reliably, we required a minimum coverage threshold and a coverage-dependent allele frequency cutoff.

The coverageBed output was filtered with a custom script to calculate coverage by gene for all bases with a minimum coverage threshold requirement of three reads.

The E-value threshold was set at 1E-3 for MSA generation and the minimum coverage threshold for MSA hits was set at 20%.

In order to assure sufficient power to detect variants, the minimum coverage threshold of a coding base was set to 30.

This way the variation in read number per sample could have been minimized and possibly more individuals would have passed the minimum coverage threshold.

We also report the percent of exonic bases reaching a minimum coverage threshold of ≥20×, a level of coverage depth necessary to call heterozygous SNVs with approximately 99 % sensitivity in WES and WGS data [ 25, 26].