It covers only 13percentt of the merged zoning lot from which it derives its development rights, where the new rules would require a minimum coverage of 33percentt.
Mapping of these SNPs was undertaken using 90% of sequence similarity and minimum coverage of four reads at each SNP position.
Plan criteria included minimum coverage of 144 Gy to the planning target volume (PTV), ≤ 70% dose to 150% of the PTV volume (V150-PTV), and the quantity of needles ≤ 70% of the size of the PTV, in cc.
Minimum coverage of six sequence reads at the SNP locus.
The distribution had a minimum coverage of 5.32% and maximum coverage of 5.42%, and a mean of 5.37±1e-6 5.37±1e-6%
In total, we called between 2405 and 3371 SNPs per sample requiring a minimum coverage of ten (Table 4).
We sequenced all of the 24 specimens to an average coverage of 1079× and a minimum coverage of 600× per exon and specimen.
With this coverage threshold we correctly identified 97.9% of dbSNPs, 6.6% better compared to when a minimum coverage of 4 fold was used.
Furthermore, we required that at least 90% of the 10,000 base pairs downstream of the SNP have a minimum coverage of 8 in the full data set.
For SNP detection we maintained the same parameters as for the full dataset, but with a minimum coverage of 5 and at least 2 reads carrying the variant allele, to compensate for the reduced coverage introduced by the simulations.
For SNPs other than the high confidence SNPs we required the following criteria: minor allele count >1, a minimum coverage of 4 and a minimum base quality of 20.
