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The minimum coverage needed for reliable variant calling was set at 20×, and regions above this threshold (well covered regions, WCR) were identified in all samples related to the same primary tumor (primary related samples, PRS).
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When considering that individually targeted interventions are implemented effectively, the minimum vaccination coverage needed to achieve herd immunity is reduced.
From this condition we derived the reduced minimum vaccination coverage needed for outbreak control and compared it with the standard vaccination coverage needed for achieving herd immunity (coverage>1 – 1/R).
In this study, the minimum read coverage needed to robustly identify an expressed exon-exon junction was determined using information derived from the experimental validation of a select group of potential strain-specific exon-exon junctions and the overall median exon-exon junction read coverage, while potential alignment artifacts were reduced by focusing on only canonical splice sites.
In addition to our 454 and Sanger comparisons, we used the deep 454 sequence coverage (average 1300×) and computer simulations to estimate the minimum amounts of coverage needed to detect homoplasmic and heteroplasmic mtDNA variants.
Conversely, 100× average depth of coverage is commonly referred to as the minimum amount of coverage needed in clinical applications, regardless of the total amount of sequence data obtained.
In an attempt to minimize the impact of potential sequencing errors on our identification of SSS events, the next task was to determine the minimum exon-exon junction read coverage needed to reliably detect an expressed splicing event.
If your policy provides minimum coverage, you may need to compute the value of the loss or damage of a newer vehicle (the rental car) and decide if you need to supplement your coverage.
Coverage needs: none.
Coverage needs: Wi-Fi or data.
Coverage needs: Wi-Fi or data. .
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