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Glandular cells were finally collected in 30 μm meshes with minimum contamination of non-glandular trichomes.
The approach developed for recovering bacteria from infected cells ensured the minimum contamination of extracted bacterial RNA with eukaryotic RNA.
To pave the way for obtaining CTC-derived molecular signatures and functional readouts, it is important to develop novel methodologies that can recover CTCs with minimum contamination of white blood cells (WBCs) and negligible disruption to their viabilities.
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The goal of the minimum contamination problem is to minimize the expected size of contamination by removing a set of edges of at most a given cardinality [27].
No filling of the uterus with extender immediately prior to NS as described originally for the minimum contamination technique [ 7] was done, to allow for the confirmation of ejaculated semen in the uterus by ultrasound scanning.
Pumped out approximately 30 L of fluid in <130 min and collected formation fluid (water) with minimum contamination (Fig. 8b).
Minimum contamination level.
There is a small deviation in the difference between minimum contamination and maximum contamination.
The composition of the infiltration solution had been optimized by varying the KCl and Triton X-100 concentrations to obtain maximum protein with minimum contamination (see below).
Therefore, artificial insemination (AI) associated with minimum contamination techniques is considered to have a distinct advantage over natural service to control the transmission of these bacteria [ 3].
To rule out false-positive results due to contaminating plasma cells, the sensitivity of the PCR system to detect IGH rearrangement in EPCs was evaluated by quantitating the minimum contamination with tumor DNA required for IGH gene rearrangement.
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