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A minimum cell viability of 66% following the overnight rest was required for assay by ELISpot and ICS.
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The cell suspension cultures maintained a minimum 65% single cell viability for 6 months when the cell suspension were sub-cultured as described above.
In a preliminary study, we found that this dose of BSO is capable of increasing the intracellular levels of ROS, assessed using DCF-DA, with no or a minimum effect on cell viability.
EUK-134 (Cayman) and LA (Sigma) were most effective in cultured fibroblasts at 100 μM and 500 μM, respectively: doses that seem to have a minimum effect on cell viability as determined by the WST-based technique (Roche Diagnostics).
Sample illumination was kept to a minimum to prevent perturbing cell viability.
Moreover, using a fast lock-in amplifier (details are given in Methods), our current imaging speed can be as fast as 3 s per frame (512 × 512 pixels), nearly 10 times faster than before, which enables time-lapse imaging in live cells with minimum phototoxicity to cell viabilities.
We show that TBP levels are sensitive to parametric changes under many of the physiological conditions and a certain minimum amount of TBP is required for cell viability.
We observed that a shift to the right indicates greater stringency as the minimum level of transcript needed to sustain 50% cell viability increases.
Cultures were treated concurrently and cell viability was evaluated (means ± standard error, minimum of n = 3).
Cultures were treated concurrently with varying concentrations of ZnO NP for 24 h and cell viability was evaluated (means ± standard error, minimum of n = 3).
Cell viability was determined by Trypan blue exclusion on a minimum of 200 cells using a Countess automated cell counter (Invitrogen).
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