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Minimum cell dose required for the CD34+ enriched progenitor cell graft was 2 × 10 cells/kglls/kg.
Here we initially confirm the observations of several other groups; plerixafor+G-CSF is an effective first-line strategy, whereby 78% mobilise at least 4.0 × 10 cells/kglls/kg (71% in 1 or 2 aphereses), and 96% mobilise a minimum cell dose of at least 2.0 × 10 cells/kglls/kg.
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In this current analysis we used a CD34 yield of 2 million CD34 cells/kg as the minimum cells dose required to perform a single ASCT and 4 million CD34 cells/kg as the ideal collection required for a single transplant for MM or lymphoma.
The target cell dose selected is lower than minimum threshold cell doses previously reported by other investigators in two-unit studies in adults treated with non-myeloablative conditioning.
The ASCT conditioning regimen was melphalan 200 mg/m on day −1 followed by infusion of G-CSF-mobilized peripheral blood stem cells (minimum dose 2 × 10/kg) on day 0. Deferred ASCT was performed using cells mobilized and collected prior to the initial ASCT.
Any activin A secreted by LE6 cell cultures was below the minimum detectable dose of the ELISA kit (data not shown).
Five objective responses were observed in 16 patients (13 multiple myeloma, three mantle cell lymphoma) enrolled at the minimum effective dose or higher.
As shown in Fig. 2B, ATA prevented PR8 infection of MDCK cells in a dose-dependent manner with a minimum effective dose of 6.5 µg/ml required to protect 50% of cells (EC50).
It is an acute intestinal infection caused by bacteria of the genus Shigella, with a minimum infective dose as low as 10 100 bacterial cells.
Needle deflections of only 5° from the insertion angle decrease the minimum target dose by 10%, thus increasing the tumor-cell survival rate by a factor of 200 [ 8].
The main symptom of this infection is bloody diarrhoea and the minimum infective dose is as low as 10 100 bacterial cells due to relative resistance to stomach acid [ 1].
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