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For eubacteria, the 2.3 Ga minimum calibration (constraint), from the geologic record, was chosen because it encompasses all of the hypothesized time estimates for the origin of cyanobacteria.
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Hereby we could check whether the excluded calibration constraint was accurately estimated by the remaining ones.
Because divergence time should predate the fossil occurrence, all calibrations followed an exponential prior with the offset value set to the minimum calibration age.
Fortunately, Cetacea has an extensive fossil record (see [ 32]) permitting the use of multiple calibration constraints.
Calibration constraints (in Myr) used as priors in the BEAST analysis of cytochrome b of soricids.
Calibration constraints (in Myr) used as priors in the BEAST analysis of mammalian introns.
To exclude the possibility that individual calibration constraints may bias our dating analyses, we repeated them after removing each calibration point in turn following [ 16].
Moreover, use of relaxed molecular clocks and fossil calibration constraints also permits estimation of divergence times of clades.
It is conservative to assign a fossil to a stem node given that a hard minimum time constraint is used for calibration [ 45].
Additional file 3: Divergence ages and 95%% height posterior densities obtained with fossilized birth-death dating [ 34 ] and the DPP model using five minimum age constraints for calibration (P. Kapli, G. Grimm, unpubl. data).
The dates inferred from our study were obtained based on time calibration using the fossil taxon Archaeanthus (see Methods) as it is generally accepted to represent a minimum age constraint for the stem of Magnoliaceae [ 24, 49, 50, 53, 58, 59].
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