Sentence examples for minimum average quality from inspiring English sources

First, reads were quality filtered on read length (200 450 bp) allowing for homopolymers (maximum 8), a maximum of 2 differences with the primer sequence and minimum average quality score of 20 over a window of 50 base pairs.

Previous results obtained in a smaller pilot study [17] indicated that a minimum average quality score of 12 was an acceptable threshold, and, therefore, all sequence reads with an average quality score<12 were removed from the dataset.

As VarScan largely depends on individual base quality scores to distinguish between true SNPs and sequencing errors, we varied the minimum average quality (M.A.Q).

For pair-wise comparisons of gene expression, t-tests were performed on genes with a minimum average quality of 10 reads in at least one genotype.

All reads were trimmed with Trimmomatic software (Bolger et al. 2014) to a minimum quality score of 20 from each end and a minimum average quality of 20 using a 4-bp sliding window.

Leading and trailing bases with a quality score < 5 were trimmed from reads and then each read was trimmed by a sliding window with a width of 4 bp and minimum average quality of 15.

Based on the reported quality information, the user can choose to filter the raw data following several filters, such as maximum and minimum length of the sequences and their minimal average quality.

Parameters for SNP analysis using CLC Genomics Workbench: max # of gaps and mismatches 2, minimum average of quality of surrounding bases 15, minimum quality of central base 20, minimum coverage 1, minimum variant frequence 35%.

Prinseq-lite 0.20.04 (Schmieder and Edwards 2011) was used for quality control and filtering of reads (minimum read length of 50 nt, minimum average base quality of 25, maximum ambiguous nt (N) of 1) after which the data were used for SSR mining.

A further quality check was conducted for the final set of putative transcripts by selecting only those sequences at least 200 nucleotide long and with a minimum average sequence quality of 30 (corresponding to an average error rate of 1/1,000).

Threshold values were then applied to obtain the final set of contigs representing European eel transcripts: a minimum contig length of 200 nucleotides and a minimum average sequence quality of 30 (Phred Scores), corresponding to an error rate of 1 per 1,000 bp.