There should be a minimum amount of cells assigned for every type of material.
The minimum amount of cells that can be detected using F-based imaging ranges from 10 to 10 cells (Ahrens and Bulte, 2013).
To determine the minimum amount of cells necessary to obtain enough RNA for a stable real-time quantitative polymerase chain reaction (PCR), infiltrating lymphocytes in the LSG specimens were microdissected and used for RNA isolation, cDNA synthesis and real-time PCR.
Half maximal flocculation efficiencies (FE50) were calculated as the minimum amount of A. fumigatus cells required to harvest 50%% of the 1.0 × 10 cells/mL cells.
The idea is to preserve the minimum amount of adaptive IS cells in the body [ 44].
However, a minimum amount of seeded UCBMSCs (5,000 cells or higher) was able to drastically abrogate T cell alloproliferation, fully reaching the values of the negative control (no MDDC stimulation), while lower numbers did not show a clear immunomodulatory effect.
To determine the minimum amount of time required for cells to be subjected to a metabolic precursor to produce modified cell-surface glycans that could be detected by click chemistry, we performed a pulse-chase experiment, in which Jurkat cells were treated with Ac4ManNAl (50 μM) for 1, 5, or 10 min before cells were washed and transferred to the culture medium without the unnatural sugar.
We show that TBP levels are sensitive to parametric changes under many of the physiological conditions and a certain minimum amount of TBP is required for cell viability.
First, the initial attraction of sperm to the spermathecae within 6 hr of mating requires a minimum amount of secretion from the secretory cells (SCs).
This result illustrates once again that a minimum amount of Smn is required by all cell types [1], [34], [35], [43], [44], and this appears to be about 5% during development in mice.
