Sentence examples for minimum allele fraction from inspiring English sources

The performance of VarScan2 is closely related to the minimum allele fraction threshold, because VarScan2 will suppress any mutations below this threshold.

Though VarScan2 can achieve a higher sensitivity of 0.5 if its minimum allele fraction threshold is lowered to 0.05, it then generates a much higher false positive rate (300 false positives per Mb).

We could improve VarScan2 sensitivity after tuning the minimum allele fraction cut-off from the default 0.20 to a lower value, but at the cost of significantly compromised specificity (data not shown).

The minimum coverage was set at 10X, mutation allele fraction ≥10% and ≥5 reads that support this mutation.

For this application, we required that the interrogation site have a minimum read coverage of 10X and a minor allele fraction (maf) of 0.05.

The mutant allele fraction was estimated from Sanger sequencing across the allelic variants.

In the case where the minimum allele frequency is low, the difference between the frequencies of the two alleles at one locus is large and disparate, while the differences are much smaller when the minimum allele frequency is high.

For all positions, a minor allele fraction was calculated by taking the smaller fraction of the two counted alleles.

We hypothesize that variant allele fraction (VAF) data derived from massively parallel sequencing studies may provide further insights into the clonal architecture and pathogenesis of NPM1-driven leukemogenesis.

Deep sequencing of 78 tumor specimens (≥1000× average unique coverage across the capture region) achieved high sensitivity for detecting somatic variants at low allele fraction (AF).

All SNPs had a minimum allele frequency >5%, displayed less than 5% of missing values, and were in HWE (p > 0.05).