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Orthologs were defined as proteins that were reciprocal best matches when all proteins from both genomes were aligned, with a minimum alignment of 60% of both proteins.
A minimum alignment of at least three identical consensus sequences defined an allelic sequence from the pool of sequences.
The threshold value of identity was set to 95% with minimum alignment of at least 80% of the longer sequence.
A BlastN search was performed against the SGN Cornell unigene database (http://solgenomics.net/), using as cut-off parameters 90% identity and a minimum alignment of 100 bp.
Therefore, the same criteria for defining similar regions in the cDNA sequences (i.e. an e-value−5 and a minimum alignment of 50%) were used for constructing the orthologous cDNA platform used in this study.
A second BlastN search was made against the 16 K Fukuoka eggplant unigene dataset (in the article referred as 16 K, http://vegmarks.nivot.affrc.go.jp[ 11]), using as cut-off parameters 95% identity and a minimum alignment of 100 bp.
Similar(53)
Sequence alignments with BLAST were carried out using a threshold E-value of 10-5 and a minimum alignment coverage of 20% of the query sequence.
To estimate the divergence time in different clusters of L. casei, the minimum evolution phylogeny for the 53 strains based on concatenated sequences of the five MLST loci that could be rooted with homologous genes in the closely related species P. pentosaceus (>90% nucleotide sequence identity over a minimum alignment length of 90% of both genes) was used.
QC-passed reads were functionally annotated using the COG database, with minimum e-value of 10−5, minimum identity of 60% and minimum alignment length of 15.
The final alignment consisted of 1042 sequences, with correlations being calculated with a cutoff score of 10, minimum alignment size of 20% and Δf = 0.30.
All the alignments were carried out with a minimum alignment ratio of 0.93, and the minimum size of alignment of 20.
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