Other mass spectrometric data generation parameters were as follows: collision energy 24%35%5% for MS3 scans), MS scan range 400 1,800 m/ z, minimum MS signal intensity 500 counts, minimum MS/MS signal intensity 100 counts, and MS/MS activation time 120 ms (30 ms for MS3 scans).
Data dependent MS/MS analyses were also performed on the same instrument using an isolation width of 1, collision energy of 42%, activation Q of 25, activation time of 30 ms, minimum MS signal 1 × 10, and a repeat count of 4 prior to a 0.75 s exclusion.
Parameters applied for fragmentation were minimum signal required 1000; isolation width (m/ z) 2.0; activation time 30 ms; normalized collision energy 35.0; and activation Q of 0.250.
Tandem MS were collected in the high-pressure linear ion trap (MS/MS) with the following settings: ion threshold, 5000; minimum intensity, 3000; maximum ion accumulation time, 25 ms; and activation time, 10 ms.
Prior to test the overall performance of the motor, the performance of individual cell is tested in terms of the time delay, stroke, static friction force, and the minimum activation current.
Next, we used a different activation time to mimic the fertilization of the egg.
(5) The values of the activation time matrix are scaled so that the maximum value is equal to 64 (which correspond to blue) and the minimum value is equal to 0 (which correspond to red).
Activation times were linearly interpolated and color coded with a red color signifying earliest activation time.
The minimum activation energies required for each material are also explored.
