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Before quantitation a second filtering step was performed to retain only those proteins with enough abundance (minimum abundance of 0.001) for a low-biased quantitation [ 90, 91].
Nine automatically detected feature pairs (Table S-3) with a minimum abundance of 10 counts were selected for subsequent MS/MS experiments.
Both the native and the U-C precursor ions had to exhibit a minimum abundance of 10 counts in at least three recorded scans for being selected for successive LC-HRMS/MS measurements and evaluation by FragExtract.
To accommodate the fact that de novo assembly is unable to account for T. amboinensis chemoreceptors that yield little to no transcriptional signal (i.e., those that may be present in the genome but are otherwise not discernable), we have limited our quantitative analysis to transcripts that showed a minimum abundance of two RPKM.
Minia was compiled using the make k = 100 option to include higher k-mer sizes and run with a k-mer size of 31 and minimum abundance of 4. Mira (version 3.4.1.1) was run in accurate mode (job=denovo,genome,accurate,solexa SOLEXA SETTINGS GE:tismin=100 tismax=200) on a subset of 20 million reads randomly chosen from library 24 by a custom Python script.
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Pooled data were used to reach minimum abundances for index calculation and to fulfil the criteria of the multihabitat sampling technique of the WFD protocols.
Data utilized in the histogram was restricted to miR observed at a minimum abundance ratio level of 5 per thousand miR observed.
The Lactobacillus spp. had a minimum abundance under applied current of 5.9 % and a maximum of 67.0 %, compared to the control case with a minimum of 0.0 % and a maximum of 1.3%% (ignoring t = 0, at which both had greater than 99%% abundance of Lactobacillus spp., identical to the feed).
One limitation of this method is that it requires a minimum abundance and uptake rates of the target group in the sample (Teira et al., 2004).
Low estimates of minimum abundance (about 25 mature females) confirm that the population came perilously close to extinction during the late nineteenth and early twentieth centuries, while a low estimated growth rate (4.6%) suggests a rate of recovery slower than that reported for some other southern right whale breeding stocks.
For automatic processing, GlycReSoft 1.0 parameters were set as Minimum Abundance, 1.0; Minimum Number of Scans, 1; Molecular Weight Lower Boundary, 200 Da; Molecular Weight Upper Boundary, 6000 Da; Mass Shift, ammonium; Match Error (E_M), 5.0 ppm; Grouping Error (E_G), 80 ppm; Adduct Tolerance (E_A), 5.0 ppm.
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