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We also consider the effectiveness of regular replacement of the low-concentration iodine solution for increasing staining while minimizing tissue shrinkage.
The ability to stimulate matrix production without inducing high active tension will aid in the development of robust tissue engineered heart valves and other connective tissue replacements where minimizing tissue shrinkage upon implantation is critical.
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Embedding in celloidin was selected so as to minimize tissue shrinkage.
To minimize tissue autofluorescence, a photobleaching protocol was implemented.
When compared to other commonly used cryoprotective solutions such as 30% of sucrose, tissue shrinkage is minimized by the use of glycerin/DMSO-aqueous solutions.
The effects of tissue shrinkage after processing and sectioning distortion were minimized by ensuring optimal formalin concentration and fixation time in accordance with College of American Pathologist (CAP) best practices.
This suggests some partition-specific differential tissue shrinkage, possibly related to cell content, exactly as observed in formalin-only based shrinkage49.
All recorded surface temperatures were within the range known to be acceptable for tissue shrinkage.
Predictable tissue shrinkage during frozen section histopathologic processing for Mohs micrographic surgery.
Tissue specimens obtained from the trunk or extremities showed a greater degree of tissue shrinkage (16.3%) than specimens obtained from the head and neck (10.2%).
OBJECTIVE: To quantify the degree of tissue shrinkage in Mohs micrographic surgical specimens processed with routine frozen sections and subsequent hematoxylin and eosin staining.
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