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To perform ChIP-seq in frozen tissues while minimizing tissue handling and cross-contamination concerns, we developed an approach that uses dry pulverization.
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To minimize tissue handling, a sterile sample kit was available for each patient.
I can train a physician assistant, a masters level professional, sewing and basic tissue handling skills in about a year.
Wooden tools were used in all tissue handling.
Tissue handling induced modulations are not only confined to messengers.
The issue of quality can be easily solved using appropriate tissue handling techniques.
†While 10 cases were studied, sample 4 was excluded due to inadequate tissue handling.
Tissue handling closely resembles that used by clinicians to prepare specimens for routine pathology analysis.
Tissue handling and methods of measurement have been extensively described elsewhere [ 37].
We investigated the relative impact of several different tissue handling conditions on RNA integrity.
However, choline levels are affected dramatically by agonal status and postmortem delays in human tissue handling.
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