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Recording and manipulating neural activity in awake behaving animal models requires long-term implantation of cranial implants that must address a variety of design considerations, which include preventing infection, minimizing tissue damage, mechanical strength of the implant, and MRI compatibility.
Yet, knowledge of their material behavior is paramount across a spectrum of biological and engineering applications from minimizing tissue damage in ultrasound and laser surgeries to diagnosing and mitigating impact injuries.
Apoptotic cell loss may be an attempt by the host stroma to escape necrosis, thereby minimizing tissue damage.
These data highlight to the importance of minimizing tissue damage and consequent peritoneal inflammation even when the tumor burden is relatively minimal.
Our objective was to develop and refine a protocol to maximize electroporated DNA expression in skeletal muscle while minimizing tissue damage.
We have attempted to develop an exact protocol to maximize DNA expression while minimizing tissue damage following EP of skeletal muscle in vivo.
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To enable microcirculatory assessment, a 2-cm skin incision was performed on the medial side of the left hind limb, and the perimysium was carefully separated from the muscle by blunt dissection in order to minimize tissue damage and bleeding [7].
Repeated reentry and readjustment of the needle position were avoided to minimize tissue damage and leakage of cells.
We used the pipettes with fine tips (5 10 µm diameter) to minimize tissue damage that might label the fiber of passage.
To minimize tissue damage, the final position of the probes was reached slowly, in not less than 20 min. Perfusion of artificial cerebrospinal fluid, containing 144 mM NaCl, 3 mM KCl, 1 mM MgCl2, 2 mM CaCl2 (pH 7.4), was performed at a rate of 1 µl/min.
Despite the encouraging results much can still be done to minimize tissue damage during the cryopreservation procedure.
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minimize tissue damage
minimise tissue damage
reduce tissue damage
reduced tissue damage
minimizing tissue motion
minimizing tissue shrinkage
minimizing formation damage
minimizing styling damage
minimizing tissue hysteresis
minimizing conductivity damage
minimizing reservoir damage
minimizing chemical damage
minimizing sample damage
minimizing liver damage
minimizing tissue oedema
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