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Furthermore, with count data, summing across technical replicates provided a superior method for minimizing technical variation compared with calculating an average value.
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We applied stringent quality control and filtering procedures to minimize technical variation.
This dataset was highly idealized because the relatively small number of replicates was carried out in such a way as to minimize technical variation.
To minimize technical variation, samples were randomly assigned to lanes, barcoded, and multiplexed at 12 24× per lane, and two to four technical replicates for each DO sample were sequenced.
A different sequence of antibody staining was used to minimize technical variation and duplicate blots were run to make sure that the AMC detection was not influenced by previous detections or multiple stripping steps.
Development of a single robust RNA isolation protocol for all grapevine tissues can minimize technical variation when comparing real-time RT-PCR experiments or other downstream applications such as microarray analyses.
While both approaches are well established for comparative proteomics as they minimize technical variation by mixing samples prior to analysis, there are a number of challenges hindering their development and implementation on a wide-scale basis.
Conscious of the need to minimize technical variation within datasets while retaining as much information as possible, we then explored three sets of probes which we expected to provide performance metrics that could be used to evaluate processing and normalization methods: probes in iDMRs, SNP probes, and CpG sites on the X-chromosome.
The research community requires a consensus on how to implement these bioinformatics tools in a way that finds the middle ground among minimizing technical errors and tag switches, maximizing the sensitivity to biological variation, and downstream reanalysis requirements.
Improved FPs facilitate life science research by minimizing technical hurdles that otherwise complicate their use in imaging applications.
We observed minimal technical variations among the technical replicates of each sample.
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