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By including a separation step prior to the MALDI-TOF-MS analysis and hereby minimizing suppression effects allowed us to obtain higher sequence coverage of proteins compared to conventional MALDI sample preparation methodology.
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With this approach it may be possible to disclose more detailed information and minimize suppression.
Clean samples are essential for minimizing ion suppression and matrix effect in UPLC MS/MS analysis.
Because of the frequently low stoichiometry of PTMs and the ion suppression effect from unmodified peptides of high abundance, phosphopeptides and glycopeptides have to be enriched before MS analysis to minimize such suppression [9].
Clean samples are essential to minimize ion suppression and matrix effect in bioanalytical method.
The pulsed Mims ENDOR spectra were recorded with different pulse delays τ between the first two π/2 microwave pulses and subsequently added to minimize the suppression effects.
Maintenance treatment with steroids should be given as an early morning dose to minimize adrenal suppression.
Electrospray ionization mass spectrometry (ESI-MS -based protESI-MS -basedabolomic analysESI-MS -basedemical seproteomic prior to ionizandon to add selectivity, reduce metabolomicanalysesstion, and minimize ionization supprelyion at the elechemicaly separations
Higher charge state ions usually generate more effective ETD fragmentation; therefore, formic acid instead of TFA was used in the mobile phase to increase charge state for more effective ETD fragmentation and to minimize ion suppression.
In order to evaluate such low concentrations, it is very important to minimize ion suppression, in particular in the chromatography method coupled with mass spectrometry or tandem mass spectrometry.
We minimized data suppression issues by regional aggregation of cases, yet for some of the race/ethnicity categories, low cell counts, particularly during the reference period, and underreporting combined to mask some of the relevant RR calculations.
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