While we tried to minimize potential errors by increasing the number of SpO2 values collected, we are aware that potential artifactual SpO2 values or ones resulting from acute events unrelated to the disease process may have been included (such as patient ventilator asynchrony, obstruction of endotracheal tubes, suctioning and hemodynamic instability).
First, they provide a more "realistic" (i.e., 3D vs. 2D) representation of zebrafish swimming activity, minimize potential errors in interpreting fish lateral movements (towards the camera/observer), and simplify comparison of manual, event-based and automated endpoints for each fish within a single track file.
Then, to minimize potential errors in contig assemblies, sequence alignments of each PUT contig were visually inspected.
We chose a cut off of Ks = 1 to minimize potential errors associated with multiple hits (Li 1997) and potential multiple relocalizations at higher Ks values.
To minimize potential errors associated with the bioelectrical impedance measurements, we provided standardised conditions (supine position, resting, fasting) for the measurements.
A large number of elements were used in the linear ED mesh to minimize potential errors due to the mapping of data.
These amplicons were generated using Pfu Turbo DNA polymerase (Stratagene Inc., Carlsbad, CA) in order to minimize potential errors generated during PCR reactions.
Due to the short length and high variability of the sequences, the TFP superfamily is not an ideal candidate for sequence-based phylogenetic analyses, but the wealth of information that it could provide justifies the exercise, as long as measures are taken to minimize potential error and the relevant caveats are taken into account.
To minimize potential error, all RAs involved with this study were trained to complete the PEDI by an occupational therapist.
Considering the maximum threshold of 5% of genotyping errors in population size estimation [ 49], it could be one means to minimize potential error sources by reducing the number of microsatellite markers used.
