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These observations suggested that the scs/scs' insulators are minimizing position effects.
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To effectively minimize position effects the transgene must be bracketed by insulator elements.
Pots were arranged randomly in trays to minimize position effects.
To minimize position effects, cases and controls will be randomly distributed.
Flanking transgenes with insulator elements has also been attempted to minimize position effects and insertional mutagenesis.
Flanking H19 insulator sequences were added to minimize position effects (see extended methods for details).
These variants were all inserted into the same genomic locus to minimize position effects on levels of transcription.
Plants were fertilized once a week with Peters 20-10-20 fertrayser and trays were rotated every 2 days to minimize position effects.
Trays were rotated 180° and moved to a new shelf every other day to minimize position effects within the growth chamber.
Another important consideration is that flanking transgenes by TAD boundaries could likely insulate their expression from the influence of neighboring control elements and minimize position effects.
To minimize position effects we have created a set of piggyBac transformation vectors that contain either the scs/scs', gypsy or chicken β- globin HS4 insulators.
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