Exact(4)
We used forebrain astrocytes and cerebellar neurons because in our experience the forebrain and cerebellum yielded very high numbers of astrocytes and neurons, respectively (thus minimizing animal use).
Embryonic stem cells (ESCs) offer an excellent model to identify TUFs because developmental processes in vivo can be recapitulated in vitro to a certain extent in a time- and cost-effective manner while minimizing animal experiments.
By titrating the amount of tamoxifen to maximize recombination while minimizing animal lethality, we determined that 30 μg tamoxifen/g body weight/day injected on three consecutive days is the optimal condition for the αMHC-MerCreMer system to induce recombination in the Rosa26-lacZ strain.
The proposed USDA 2015 dietary guidelines recommends choosing plant based sources proteins wherever possible, and minimizing animal sources of protein, especially those that are processed or that contain significant percentage of calories from saturated fat.
Similar(56)
Therefore, alginate 3D scaffolds may facilitate our understanding of tumor cell behavior, malignancy, ultimately improve the quality of in vitro drug screening, pre-testing clinical treatments and minimizing animal-based experiments.
All efforts were made to minimize animal suffering and to use the minimal number of animals necessary to produce reliable results.
All efforts were made to minimize animal suffering.
All efforts were made to minimize animal suffering and the number of animals that were used.
All efforts were made to minimize animal suffering and reduce the number of animals used.
Every effort was made to minimize animal suffering in each experiment.
Larger piospheres should also be used to minimize animal impact per unit area.
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