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The nutrient-limited PFM agar was used to ensure standardized conditions across sampling dates and substrate types, because it minimizes growth of naturally associated microbes.
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Cytosine arabinoside (final concentration 10 μM; Sigma Aldrich, Munich, Germany) was added at day 1 and day 3 to minimize growth of non-neuronal cells.
The aim of this experiment was to produce Se-enriched Chlorella biomass in longer run with high amount of Se incorporated into biomass while minimizing growth inhibition and Se losses.
Given the range of habitats the environmental isolates came from, tryptic soy broth was used minimize growth medium-related effects.
For differentiation and proliferation analyses, animals were sacrificed at exactly postnatal day 21 to minimize growth variation.
Cytosine arabinoside (final concentration 10 µM; Sigma Aldrich, Munich, Germany) was added at day 1 and day 3 to minimize growth of non-neuronal cells.
Modifying salsa recipes to include growth-inhibitory ingredients might limit amplification of Salmonella [22]; all fresh salsas with a pH ≥4.2 should be stored in adherence with established time and temperature recommendations to minimize growth [24].
600,000 cells were plated per dish and cultivated in Neurobasal medium supplemented with 1% (v/v) B27 supplements (Gibco) and 0.25% (v/v) 200 mM L-glutamine (Gibco) to minimize growth of astrocytes and microglia.
BMW media is designed to minimize growth differences between different species [ 21].
We therefore first optimized our growth medium to minimize growth differences between species ('Media tests' under 'Materials and methods').
To detach MDA-MB-231 and MCF-7 cells, cultures were trypsinized for 3 min to minimize growth inhibition, and detachment was monitored with a phase-contrast microscope.
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