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We employed thermal-shift assay for high-throughput screening of 192 conditions to identify optimal pH and salt conditions that increased the stability and minimized aggregation of AurB69 333.
Inclusion of salts/buffering agents has resulted in increased solubility and thermal stability, and minimized aggregation of proteins.
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The glycerol in the buffers minimized aggregation, and the high concentrations of imidazole and KCl were crucial to improve the accessibility of TEV protease to the TEV cleavage site and thereby increase the yield of the cleavage reaction.
Sellers would get a better price, through aggregation of demand.
Politics is driven by aggregation of power and privilege.
The Aggregationr is used tofselect the minimum membership values outputsthe three input membership values.
Mr. ARRINGTON: Aggregation of like Facebook inbox.
This indicates aggregation of TSNP.
Phase 5. Aggregation of judgments.
Significantly, we observed that the presence of the ATPase-deficient Kar2 cysteine-substitution alleles (Kar2-C63D/F/Y/W) dilutionilutionotonlynlessenedned aggregation (minimized light scattering) of denatured rhodanese but also was more effective than wild-type BiP at minimizing rhodanese aggregation.
Her work focuses on spawning aggregations of Nassau grouper.
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