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Embedding in celloidin was selected so as to minimize tissue shrinkage.
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We also consider the effectiveness of regular replacement of the low-concentration iodine solution for increasing staining while minimizing tissue shrinkage.
We found that minimal tissue shrinkage occurred during this overall process (on average, tissue measurements increased by 6.1% after paraformaldehyde fixation and decreased by 6.8% after paraffin embedding).
To minimize tissue autofluorescence, a photobleaching protocol was implemented.
When compared to other commonly used cryoprotective solutions such as 30% of sucrose, tissue shrinkage is minimized by the use of glycerin/DMSO-aqueous solutions.
The effects of tissue shrinkage after processing and sectioning distortion were minimized by ensuring optimal formalin concentration and fixation time in accordance with College of American Pathologist (CAP) best practices.
Low concentrations were also preferred to reduce the possibility of tissue shrinkage observed at higher concentrations (e.g., 20%% w/v; [ 12]); lower concentrations (e.g., 3.75 % w/v I2KI) have been proposed to minimize shrinkage [ 6].
All recorded surface temperatures were within the range known to be acceptable for tissue shrinkage.
The morphology of the different TGs was in general good, though tissue shrinkage was observed in some of the TGs.
These procedures entail different degrees of tissue shrinkage complicating direct comparisons of measurement results across laboratories or articles.
The advantage of this method is that it is not affected by tissue shrinkage [99] [101].
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