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Each sample was arrayed in triplicate to minimize tissue loss and overcome tumor heterogeneity.
The authors also used the "crushing technique" of parenchymal transection, which does not necessarily minimize tissue loss and tumor exposure.
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To minimize tissue autofluorescence, a photobleaching protocol was implemented.
The original H&E stained slides were reviewed by study pathologists and representative areas of each tumor were arrayed to the triplicated blocks to minimize the tissue loss and overcome the heterogeneity of the tumors.
Apoptotic cell loss may be an attempt by the host stroma to escape necrosis, thereby minimizing tissue damage.
To decrease error introduced by sampling and to minimize the impact of tissue loss during processing, duplicate tissue cores per specimen were arrayed on a recipient paraffin block.
The Pinpoint Slide DNA Isolation System (Zymo Research Corp, Irvine, CA) is a matrix capture method which allows lifting of tissue from the slides with ease and minimizes the potential for tissue loss and contamination from flaking of tissue during scraping of the slides (http://www.zymoresearch.com/dna-purification/genomic-dna/solid-ffpe-tissue-dna/pinpoint-slide-dna-isolation-system).
To minimize any sampling error and to reduce the impact of tissue loss during processing, duplicate tissue cores for each specimen were arrayed on a second recipient paraffin block.
Vertebrates possess a striking ability to minimize blood loss after tissue injury.
Red areas show the greatest tissue loss.
Maximum projection images were obtained from image stacks recorded from 14 μm testis tissue sections to minimize signal loss due to sectioning.
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