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The worms were collected from the sediment and placed in beakers with 500 mL of new artificial freshwater for 6 hr, a period shown to allow these organisms to purge > 98% of their gut content but also minimize tissue depuration of non-polar hydrophobic chemicals (Mount et al. 1999).
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To minimize tissue autofluorescence, a photobleaching protocol was implemented.
To minimize tissue handling, a sterile sample kit was available for each patient.
Each sample was arrayed in triplicate to minimize tissue loss and overcome tumor heterogeneity.
Embedding in celloidin was selected so as to minimize tissue shrinkage.
At least 2 biopsies from the colon were used per individual to minimize tissue heterogeneity.
All tissue pieces were divided in two and pinned to small rectangles of cork to minimize tissue distortion.
Biopsy of suspicious lesions are often approached stereotactically, thus minimizing tissue injury.
Biodistribution in normal animals was characterized by high initial blood, lung and liver uptake, fast blood and soft tissue depuration and preferential excretion via the hepatobiliary system.
Surgical wound closure directly opposes the tissue layers, which serves to minimize new tissue formation within the wound.
However, all tissue samples except one were collected within 5 hours post mortem to minimize the tissue degradation.
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