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Oat endosperm was squeezed out from grains (from which the embryo and scutellum had first been removed to minimize tissue contamination) at mid-stage of development during active endosperm oil deposition (approximately 14 days post anthesis).
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To minimize maternal tissue contamination and increase the confidence of imprinted gene identification, different approaches were carried out in the two studies.
A cyclone separator tube adapter attached to the vacuum port was used to collect the E. necator tissue from the surface of the leaf to minimize plant tissue contamination.
Each biopsy was visually inspected to minimize non-inflammatory synovial tissue contamination.
To minimize microbial contamination, plant tissue was soaked in 0.5% sarksoyl for 3 5 min and rinsed thoroughly before isolating chloroplasts following the high salt protocol that does not include treatment with DNase [ 12, 25].
All other statistical analyses of the data were also carried out in R. Before normalization, all genes that showed 1.5 times higher expression in hypopharyngeal gland tissue compared to brain tissue were discarded to minimize possible contamination effects as in Whitfield et al 2003 [ 6].
To minimize contamination of other tissue types in the ventral body wall muscles, only ventral body wall muscles between A3-A5 were used for RNA extraction.
To minimize contamination and resected tissue volume, we developed a new technique without perforation termed non-exposed endoscopic wall-inversion surgery (NEWS), and present here the preliminary results.
To minimize contamination from surrounding tissue, 3 saturation bands, each 25-mm thick, were placed over chest wall muscle and liver.
Therefore, to accurately profile single cell genes from a heterogeneous cell solutions or a tissue sample, it is essential to minimize RNA contamination from surrounding cells by enriching the fraction of the target cell type.
According to our modified protocol, the tissue was processed in order to minimize microbial contamination and to remove subcutaneous elements as described earlier [ 18].
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