Sentence examples for minimize tissue autofluorescence from inspiring English sources

To minimize tissue autofluorescence, a photobleaching protocol was implemented.

Bcl-2 was measured using a channel with emission maxima above 620 nm, in order to minimize tissue autofluorescence.

Target protein antigenicity was measured using a channel with emission maxima above 620 nm, in order to minimize tissue autofluorescence.

To identify the conditions that best preserve tissue morphology and cause minimal tissue autofluorescence, we tested different alcohols.

Apart from the aforementioned properties, AOI987 absorbs and emits in the near infrared (NIR) fluorescence spectrum thus minimizing the impact of tissue autofluorescence and light-scattering that would be otherwise observed from dyes with a shorter absorption and emission spectrum.

To detect THK523 fluorescence, quenching was first performed whereby sections were first deparaffinized and tissue autofluorescence was minimized by treatment of sections with 0.25% KMnO4 phosphate-buffered saline (PBS) for 20 minutes prior to washing in PBS and incubation with 1% potassium metabisulphite/1% oxalic acid/PBS for 5 minutes.

Partly, it was due to the excitation wavelength, 740 nm, at which the imaging penetration depth was maximized, as the tissue scattering and absorption were minimized [ 22]; and also due to the emission wavelength at 385 nm, where the two-photon-excited skin tissue autofluorescence was relatively low [ 23].

By means of spectral unmixing, tissue autofluorescence was removed from the images.

Tissue autofluorescence spectra tend to be wide and strongly overlapping (Wagnieres et al. 1998).

Fluorescence techniques additionally suffer from tissue autofluorescence, resulting in an unwanted background signal.

Mice were maintained under a low-pheophorbide diet (C 1039; Altromin, Lage, Germany) to reduce tissue autofluorescence.