Another approach involves designing more intelligent CAR constructions to minimize the off-target effects.
The key challenge in the electron optical design of the MBSEM is to minimize the off-axial aberrations of the lenses.
The present study aims at proposing an innovative phase control methodology for semi-active tuned mass dampers (SA-TMDs) that intend to minimize the off-tuned problems associated with passive tuned mass dampers (P-TMDs).
To prevent this and therefore minimize the off-target effects of the recombination system, the repeated FRT-like sequence should be used as one of the counter-selection targets during evolution of a Flp variant.
Thus, we removed the genome-nonspecific probes in order to minimize the off-target probe pairing.
The siRNAs against GSK3 β were a group of four different siRNAs designed to minimize the off-target effects (ON-TARGETplus; Thermo Scientific Dharmacon, Chicago, IL, USA).
Planar mirrors (M) are placed to direct the beam at small incident angles to minimize the off-axis aberrations from spherical mirrors (F1 and F2).
We used two pairs of p27−/− MEFs and their littermated p27+/+ MEFs to minimize the off-target effects generated during cell line establishment, one being named as p27−/−(Δ51) because of the deletion of N-terminal 51 amino acids, and the other named as p27−/− FL) because it harbored deletion of the entire coding region.
Functionalized gold nanoparticles are emerging as a promising nanocarrier for target specific delivery of the therapeutic molecules in a cancer cell, as a result it targeted selectively to the cancer cell and minimized the off-target effect.
As genetically engineered nucleases are the most promising large molecule candidates in human gene therapy, minimizing the off-target effects is the prerequisite for their application in human gene therapy.
Reducing and/or minimizing the off-target effects of the lower specificity Cas9 system is an active area of genome engineering research (Fig. 1D,E; Ran et al., 2013a; Fu et al., 2014; Guilinger et al., 2014; Tsai et al., 2014).
