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Exact(9)
RT was performed in a thermal cycler (Applied Biosystems, Foster City, CA): 25°C for 10 min, 42°C for 60 min, 99°C for 5 min, and 4°C for 5 min. All RNA samples were converted to cDNA together to minimize technical variability.
To minimize technical variability, all targets were synthesized in one round and each hybridization experiment was conducted simultaneously on slides from a single batch where possible.
To minimize technical variability, only echocardiographic series were included that could be performed during these three stages by cardiologists assigned to the echocardiography unit.
To minimize technical variability, RNA processing steps (RNA extraction, probe labelling and chip hybridization) were performed in parallel for control and asthmatic samples.
To minimize technical variability, SNS-Cre/TdTomato (total, IB4+, IB4-) and Parv-Cre/TdTomato neurons were sorted on the same day.
To minimize technical variability, target synthesis and hybridizations were done in batches of 6 where all treatment groups were equally represented.
Similar(51)
This helped minimize technical noise associated with oligodeoxyribonucleotides labeling efficiency.
This selection procedure was performed twice to minimize technical errors and false positives.
Reciprocal dye swap replicate hybridizations were performed to minimize technical noise.
An effective normalization technique should minimize technical and experimental bias without introducing noise.
We applied stringent quality control and filtering procedures to minimize technical variation.
More suggestions(15)
minimize regional variability
minimize biological variability
minimize technical bias
minimize interlaboratory variability
minimize experimental variability
minimize technical noise
minimize genetic variability
minimize technical batch
minimize such variability
minimize diurnal variability
minimize methodological variability
minimize technical error
minimize cyclic variability
minimize analytical variability
minimize environmental variability
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