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This initial alignment was adjusted manually in BioEdit v.7.0.5.3 or Geneious Pro v.5.6 to minimize steps in the most parsimonious trees (e.g. Koch et al. 2010).
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The structures were energy minimized (steps = 100, step size = 0.2 Å) using rotamers derived from Dunbrack library [22] and the charges were computed using Antechamber method [23].
In the program used for this study (Strand7), coarse models are calculated by minimizing steps required to transition to the maximum element size (which is determined by the initial surface mesh), whereas fine models are built without much constraint on numbers of elements with maximum size.
Following the minimizing step, the complexes were submitted to molecular dynamics simulations (MD).
The Capto® MMC resin is a multimodal cation exchanger that does not require buffer exchange and was chosen to help minimize purification steps.
In order to minimize iteration steps with sufficient confidence regions, the Brent's method was chosen as the search method [ 40].
To minimize coupling steps and maximize yield, Fmoc- β-Ala 3-OH and Fmoc- β-Ala 3-OHere synthesized prior to SPPS (Supporting InFmoc- β-Ala 3-OH
We used two strategies to minimize this step.
Clearly, one might consider other things to minimize in step (b), such as curvature variation.
Minimizing the steps required in joining the conference truly should help in terms of keeping driver focus as much as possible on the road itself.
A primary objective is to make things super easy for investors, matching them with the right companies quickly and minimizing the steps they take when investing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com