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The block of tissue was trimmed and coated with silver paint to minimize specimen charging during imaging.
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Modifications were made to the apparatus in order to minimize wave distortions at the interface between the specimen and the bar, and to the specimen design to minimize specimen geometry effects.
All samples were stained in parallel to minimize specimen variation and cell staining intensity was graded by a masked observer.
The binding energies were corrected for specimen charging by referencing C ls to 285 eV.
Although some specimen charging did occur, the addition of 10 nm of gold to the surface (via plasma magnetron sputter coating) was sufficient to eliminate most charging effects.
An advantage of this type of product is that the specimen can be collected directly into the stabilization tube, minimizing specimen handling and immediately stabilizing the nucleic acid.
In order to minimize static charging effects, all specimens were coated with gold prior to analysis.
A low energy electron flood gun was used for all measurements to minimize sample charging.
Prior to the SEM observation, the coating was sputter-coated with platinum to minimize surface charging.
The EDS spectra were acquired at 3 kV to minimize charging and excitation volume.
To minimize charging problems, samples for SEM were coated with thin Au layers.
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CEO of Professional Science Editing for Scientists @ prosciediting.com