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The scientists also wanted a flat area to minimize secondary effects of the blast, and with little wind to spread radioactive fallout.
Consequently, in order to minimize secondary effects due to growth perturbations, we chose to harvest the cells after 5 days of dsRNA treatment.
First, to minimize secondary effects of chronic ischemia, we excluded individuals with major cardiovascular risk factors.
The motility and invasion assays were performed within 24 hours to minimize secondary effects of cell proliferation.
The amount of GAK on a sensor chip surface was kept as low as possible in order to minimize secondary effects such as mass transport limitation and rebinding.
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Noteworthy, inducible short-time overexpression of RBPs could be beneficial to measure direct effects of proteins on gene expression by minimizing secondary effects that may raise after prolonged alterations of expression levels (Scherrer et al., submitted).
Furthermore, we use the drugs for short periods of time, 50 minutes is our longest timepoint, thereby minimizing secondary effects.
This timepoint also represented a suitable trade-off between the need to allow sufficient accumulation of proteomic alterations directly resulting from UPF1 knockdown, while minimizing secondary effects.
In addition, microinjection of labelled protein allows imaging of fluorescent proteins within minutes of their introduction into cells, thus minimizing secondary effects due to changes in expression levels of other proteins [ 34].
Co-translation of the leader peptide disrupts secondary structures within the downstream RBS, thus minimizing secondary structure effects that arise from different combinations of genetic elements.
The oligomer was optimized to minimize secondary structural formations.
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