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To promote reliable analysis, and to minimize sampling components of variance, we wanted to ensure that a similar number of chromosomes were counted in Anatolia and in Sardinia for each of the assessed lineages.
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Values of the variance components used in the simulation were assumed known to minimize sampling variation.
To minimize sampling variability, the following steps were taken.
Simulated sample sizes were chosen to be large to minimize sampling variability.
To minimize sample variation, we used high quality RNA.
Store RNA solutions on ice to minimize sample degradation.
The method also used a disposable CO2 gas-transfer system to minimize sample-to-sample contamination.
A stratified random sampling technique was used to minimize sample variance and to increase precision.
Importantly, its geometry was designed to minimize the electric component of the electromagnetic hyperfrequency (HF) field in the center of the cavity, thus minimizing sample warming upon microwave emission.
For this reason, we minimized sample processing by staining for monocyte subsets in whole blood samples.
All the samples were run in triplicates in order to minimize the sample to sample variation.
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