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These protocols were highly efficient for detecting CBSVs compared to the existing methods and have great potential to minimize sample handling and contamination.
Furthermore, a direct "on-strip" digestion would minimize sample handling steps and the use of SDS for protein solubilization, which must be removed prior to MS/MS and can lead to further sample losses.
Individual neuron labeling with the iTRAQ reagents multiplex kit (Applied Biosystems, Foster City, CA) was performed essentially according to the manufacturer's manual but with several modifications to minimize sample handling and to adjust for the small sample volumes.
First, to capture expression profiles that accurately reflect the transcriptome at time of blood collection and to minimize sample handling artifacts, it is preferable to avoid additional processing steps as some degree of cell activation is inevitable during cell fractionation.
Therefore, the latter two extraction strategies, when compared to BD, were able to minimize sample handling and also shorten the analysis time by a factor of two while still maintaining comprehensive coverage of both polar and nonpolar metabolites.
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To circumvent this issue, a lateral flow immunoassay system was developed that measures iC3b in whole blood, plasma, and serum and avoids in vitro activation by minimizing sample handling.
Total RNA is used which minimizes sample handling.
Through examination of the carbonaceous materials, the appropriate analytical processes for sample transportation and handling were optimized to minimize sample damage and terrestrial contamination.
To minimize sample variation, we used high quality RNA.
For this reason, we minimized sample processing by staining for monocyte subsets in whole blood samples.
Compared to the conventionally used BD method in which polar and nonpolar fractions are analysed separately, the proposed method doubles the throughput and minimizes the sample handling time with comparable reproducibility.
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