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Store RNA solutions on ice to minimize sample degradation.
Column temperature was maintained at 45 °C to minimize chromatographic drift, and the autoinjector sample tray held at 4 °C to minimize sample degradation.
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The canine normal tissue samples used in this current study were harvested promptly minimizing sample degradation and bias that may negatively influence gene expression quantification.
To minimize sample variation, we used high quality RNA.
Surface water was collected in January 2000 and extracted within 48 hr to minimize bacterial sample degradation.
Fly samples and standard samples made in the water and perchloric acid sample matrix were stored on ice in the dark until use to minimize reduce sample degradation.
To minimize sampling variability, the following steps were taken.
This approach minimizes experimental variation such as sample degradation, flow rate, temperature, and laser stability.
In our study, to minimize miRNA degradation, samples were immediately processed for miRNA extraction after blood collection and stored at −80°C before qRT-PCR and RNase inhibitors were added during PCR analysis.
Eventual sample degradation, due to heating caused by the lamp, was minimized by means of a cooling system providing a laminar air flow.
In this regard, IMS could minimize such degradation during sample preparation, because matrix containing an organic solution was immediately sprayed on unthawed tissue sections.
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