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A low energy electron flood gun was used for all measurements to minimize sample charging.
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To minimize sample variation, we used high quality RNA.
Store RNA solutions on ice to minimize sample degradation.
To minimize sampling variability, the following steps were taken.
For this reason, we minimized sample processing by staining for monocyte subsets in whole blood samples.
A sputtered 6 nm layer of gold was deposited on top of the self-standing samples to minimize charging effects under microscope electron beam.
Lyophilized blank or loaded gelatin nanoparticles and NiMOS samples were sputter coated with gold-palladium to minimize surface charging and evaluated according to their surface morphology with a Hitachi S-4800 (Pleasanton, CA) field emission scanning electron microscope.
In fact, these samples were carbon coated for providing conducting surface to minimize the charging effect while recording SEM images.
Samples for Scanning Electron Microscopy (SEM) were prepared by drop casting the sample onto an aluminium stub and coated with platinum prior to imaging to minimize surface charging.
To minimize charging problems, samples for SEM were coated with thin Au layers.
In order to minimize static charging effects, all specimens were coated with gold prior to analysis.
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