To minimize mRNA quantification errors, genomic DNA contamination biases and to correct for inter-sample variations, we used 18s ribosomal RNAs (rRNAs) of Hb as an internal control.
To minimize mRNA quantification errors, genomic DNA contamination biases and to correct for inter-sample variations, we used 18s ribosomal RNAs (rRNAs) of P. murrayi as an internal control.
3) We adopted a novel strategy to prepare the tissue-specific cDNA libraries that relies on linear amplification of mRNAs, minimizing the quantification error rate due to limited starting material, providing high-quality transcriptome and 3'end data in the same experiment [ 37]; and 4) replaced the microarray step with Next Generation sequencing (Illumina HiSeq) to improve data resolution.
A relatively large feature size of 21 μm (6 × 6 DMD pixels) is used to minimize image analysis related quantification errors.
Furthermore, the normalization of all of the signals to the Fh100 standard, which will have a similar relative proportion of signal from satellite transitions as the rest of the spectra, minimizes the overall quantification error associated with these effects.
To maximize spatial resolution in SPECT, thereby minimizing partial volume effects, and to reduce quantification errors, distance-dependent collimator resolution and sensitivity need to be taken into account in image reconstruction (i.e. resolution recovery methods).
Figures 2b, 3b, and 4d show box plots summarizing quantification errors for different phantom scans.
As a result, quantification errors due to misregistration stayed also small in these regions.
Overall relative quantification errors were below 7% in various acquisition conditions.
The resulting quantification errors were about 20% for the largest sphere.
