Exact(1)
Secondly, nested PCR was performed at two positions on the LTR to minimize primer interference.
Similar(7)
To minimize primer dimer in high multiplex PCR, each 3′ target specific sequence was selected to minimize potential cross hybridization with other primers.
PCR primers were designed with Primer3 software, manually selecting regions with few cytosine bases in order to minimize primer degeneracy.
Multiple alignments of previous and recent H5N1 strains were performed to minimize primer mismatch.
Amplification efficiency and primer interference were checked using standard curves.
Our new primer design scheme minimized the primer-primer dimerisation and enabled the primers to use the PCR products as the template.
Some corrective actions, to minimize this interference, are proposed.
Most of the existing work on topology control is to minimize the interference or delay separately.
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