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Identical chamber and flow rates were used for all reported measurements to minimize experimental variability and coral nubbin size was kept as similar as possible (typically 1 2 g with 0.8 1.2 mg protein/coral).
To minimize experimental variability, we used the automated second derivate maximum estimation method to determine the amplification crossing point (Cp or threshold cycle) that marked the cycle at which fluorescence of the sample became significantly different from the baseline signal.
To minimize experimental variability, each sample was analyzed in triplicate and the mean expression level was calculated.
The 28-subject study was designed to distinguish between biological and experimental variability, and to minimize experimental variability.
To minimize experimental variability and loss of cell viability, all experiments were performed on fresh tumor cell suspensions prepared shortly before flow cytometry.
In order to minimize experimental variability, all steps in tissue dissection, RNA isolation and microarray operation were performed by a single investigator.
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To minimize experimenter variability, a single individual performed all tests.
This calibration has been shown to minimize experimental effects [22].
All samples were processed simultaneously to minimize experimental variation.
Successful microarray experiments are reliant on sufficient care being taken to minimize and account for experimental variability.
Study design and analysis with a mixed-effects model minimized potential confounding and experimental variability.
More suggestions(15)
minimized experimental variability
minimise experimental variability
minimize regional variability
minimize experimental time
minimize biological variability
minimize experimental loss
minimize experimental effort
minimize genetic variability
minimize experimental error
minimize experimental variation
minimize experimental bias
minimize cyclic variability
minimize experimental noise
minimize analytical variability
minimize environmental variability
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