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The Agilent microarray platform features the direct end-labeling and profiling of mature miRNAs from total RNA without any size fractionation or amplification to minimize experimental loss, bias, or variations [ 31, 32].
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To minimize experimental variability and loss of cell viability, all experiments were performed on fresh tumor cell suspensions prepared shortly before flow cytometry.
This calibration has been shown to minimize experimental effects [22].
All samples were processed simultaneously to minimize experimental variation.
To minimize experimental variations, the same numbers of plants were grown on the same tray.
The time course samples were collected out of series and in duplicate to minimize experimental variance.
All samples were processed at the same time to minimize experimental error.
The 28-subject study was designed to distinguish between biological and experimental variability, and to minimize experimental variability.
Triplicate plates were set up for each condition to minimize experimental variations.
This observation emphasizes the importance of maintaining passage consistency to minimize experimental artifacts.
The same batches of medium constituents were used in all experiments to minimize experimental error.
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