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We used iProof high-fidelity DNA polymerase (Bio-Rad, no. 172-5300) in all PCR reactions above to minimize errors of PCR amplification, and all the constructs were confirmed by DNA sequencing.
Median values of ventilatory parameters (VT, respiratory rate, peak pressure, etc) from each patient were selected, instead of the means, for calculating the subgroups averages to minimize errors of extreme values from spontaneous ventilation or rapid intraoperative adjustments.
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These angles were modified for the current study to minimize error of marker placement by using accessible and specific anatomical landmarks.
Modifications were specifically aimed at minimizing errors of infusion rate, incorrect dose, incorrect patient, or wrong drug administered.
Clearly, there is a need to minimize errors by accurate experimental determination of the PR.
Linear amplification is expected to minimize errors in the first round of PCR.
Reputable pollsters attempt to minimize error from each of these potential sources.
We chose the assumption of a fixed evaporation of 25% of the water flux, since this has been shown to minimize error in a range of applications57,58.
This algorithm has been also shown to minimize error in the comparisons of data obtained using different experimental platforms [ 27].
By complementary transformation of the genetic code table, we have revealed its internal "yin-yang" pattern (a sub-code of sorts) that minimizes errors of tRNA recognition by putative r-aaRSs for complementarily encoded amino acids [ 19, 36].
Hucher and Sadeghi[13] found the optimal constellation parameter that minimizes error probabilities of packets for both relay and destination nodes when hierarchical modulation is used.
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