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To minimize developmental variation in gene expression we collected tissue from wandering larvae just prior to pupation.
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To minimize developmental and environmental variation, we established cohorts of equally aged females, which were all processed within a single experiment using the same batches of food algae and media.
This has the effect that as the variance among genotypes decrease due to selection, so does the variation in terms of different ability to minimize developmental errors, and hence the heritability will stay constant, or at least nearly so.
Furthermore, isolation of Bergmann glia from a restricted region of the cerebellum should minimize developmental heterogeneity between individual cells.
Fecal samples were collected during a single 1-month timespan to minimize developmental, temporal, and seasonal heterogeneity.
Gene deletion in both models was induced in adult animals, thereby minimizing developmental influences.
First, the trend we report includes only species with planktonic development, minimizing developmental type as a potentially confounding variable.
For the initial analysis of the XynA protein production in T0 clones transformed with different cassettes, we sampled young, well-developed leaves of the same size (~30 cm long, ~third-fourth leaf from top), thus minimizing possible developmental variations in XynA expression among the clones and focusing on the cassette effect.
It is filled with imperfections and developmental variation.
The differences are due not only to natural developmental variation.
An empirical understanding of normal developmental variation in the amygdala is emerging.
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