Sentence examples for minimize contaminating from inspiring English sources

We have thus consistently used lower concentrations of collagenase (0.1%) in DMEM when compared with previous studies (1 2%), and we always add 5 10% FBS to the collagenase solution in order to minimize contaminating protease activity and to protect isolated cells.

Ideally, such a procedure should (1) allow hundreds to thousands of cells to be processed rapidly; (2) ensure that no more than one cell is included in each amplification reaction yet maximize the number of cell-containing reactions; and (3) minimize contaminating DNA.

To minimize contaminating Ca2+ currents during late INa measurements, Ca2+ was omitted from the bath solution.

For preparation of genomic DNA, hESC lines were washed with PBS, released from the feeder cell layer by treatment with 1.5 mg/ml collagenase type IV and washed 3× in wash media by gravitational sedimentation to minimize contaminating MEF cells.

Our protocol compensates for the acidic nature of grape berries and introduces modifications to both increase RNA yield and minimize contaminating polysaccharides.

Animals were picked to RNAi plates using mineral oil to minimize transfer of contaminating OP50 bacteria.

To minimize breast-milk contaminating saliva, samples were collected at least 1 h after the infant's last feed.

PCR-primers were chosen to span at least one exon/intron boundary to minimize amplification of contaminating genomic DNA.

A combination of optimum dosing of trees and adoption of the tablet formulation when treating trees in sensitive habitats should minimize the risk of contaminating aquatic resources with imidacloprid.

At this point, the animals were starved for 7 to 10 days to minimize the possibility of contaminating Artemia tissue in the DNA extraction.

To minimize the possibility of contaminating parasite DNA in these PCR procedures all DNA extractions were performed in a room into which, live cultured Leishmania were never introduced.