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Plants were rotated among chambers once a week and within chambers every two days to minimize chamber effects.
Care was taken to minimize chamber opening time and gas flow was turned on immediately prior to and during chamber opening to minimize atmospheric contamination of chamber environment.
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All plants flowered and produced seed in one chamber, minimizing chamber effects on the seeds.
The experiment is conducted in an Anechoic chamber to minimize the effects of the environment.
Three biological replicates were grown in the same growth chamber to minimize experimental and sample-to-sample variation.
Injections were made through a small slit in transparent artificial dura, previously placed in the chamber, to minimize brain pulsations.
The CO2 concentrations of the two chambers were swopped, and the pots were moved between chambers and randomly re-arranged weekly to negate any possible effects resulting from the chambers and pot position within the chambers and to minimize the potential for interactive effects between the chambers and developmental stages of plants.
However, the clamping geometry of the chamber minimized stress on the sclera [ 20], and measured changes in thickness and microstructural morphology with pressure were repeatable and reversible.
To reduce the largest stress in the stress concentration regions and to minimize catalyst consumption, the regeneration chamber should be designed to give a low ratio of height to diameter.
Measurements were made on sunny days between 10 00 and 15:00 h to minimize adjustment time to leaf chamber conditions.
This low pressure will minimize structural requirements for growth chambers on missions to the Moon or Mars.
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