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In the design, a capillary tube was used to transfer cells directly into the entrance of a microfluidic constriction microchannel to possibly minimize cell sample loss.
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All T-cell samples were obtained by negative selection in order to minimize cell stimulation [ 17].
To minimize cell attachment, plates were precoated with 1 % agarose.
During this incubation, the cells were widely dispersed to minimize cell-cell contact.
The controls had been prepared in parallel using the same exome capture kits and on the same flow cell to minimize sample-to-sample variation.
The method also used a disposable CO2 gas-transfer system to minimize sample-to-sample contamination.
Cells were repeatedly washed in PSS by centrifuging, removal of the supernatant, replacing with fresh PSS to minimize the presence of cell debris and to improve the purity of the detrusor cell sample.
Each cell sample (0.5 and 5 μM KA-induced excitotoxic insult for 3 h) was subjected to 2-DE three times to minimize run-to-run variation.
Tomlins et al. also reported that LCM cell sampling in prostate cancer minimized the strong contaminating influence of stromal component and positively effected the selection of down-regulated genes [ 26].
All the samples were run in triplicates in order to minimize the sample to sample variation.
Buccal cell samples were collected from 536 control children at home using Whatman FTA Indicating Micro Cards (Cat. No.WB120211) (hereafter referred to as 'FTA cards') to maximize participation and minimize costs [ 2, 3].
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